Molecular Orientational Order Probed by Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) Microscopy: A Spectral Comparative Study - Archive ouverte HAL Access content directly
Journal Articles Journal of Physical Chemistry B Year : 2015

Molecular Orientational Order Probed by Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) Microscopy: A Spectral Comparative Study

Julien Duboisset
Pascal Berto
  • Function : Author
Paulina Gasecka
  • Function : Author
Fatma-Zohra Bioud
  • Function : Author
Patrick Ferrand
Hervé Rigneault
Sophie Brasselet

Abstract

We investigate how to extract information on the orientational order of molecular bonds in biological samples from polarized coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy. Experimentally, the mean orientation of the molecular angular distribution, as well as its second and fourth orders of symmetry, are estimated by monitoring intensity signals under a varying incident polarization. We provide a generic method of analysis of polarized signals in both CARS and SRS contrasts, and apply it to imaging of lipid bonds' orientational order in multilamellar vesicles. A comparison of the two contrasts in the lipid region around 3000 cm(-1) shows that while SRS allows retrieving pure molecular order information, CARS is generally tainted by a bias from the nonresonant contribution.
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Dates and versions

hal-01119354 , version 1 (23-04-2015)

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Julien Duboisset, Pascal Berto, Paulina Gasecka, Fatma-Zohra Bioud, Patrick Ferrand, et al.. Molecular Orientational Order Probed by Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) Microscopy: A Spectral Comparative Study. Journal of Physical Chemistry B, 2015, 119 (7), pp.3242-3249. ⟨10.1021/jp5113813⟩. ⟨hal-01119354⟩
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