Real-Time Monitoring of Chromophore Isomerization and Deprotonation During the Photoactivation of the Fluorescent Protein Dronpa
Résumé
Dronpa is a GFP-related photochromic fluorescent protein used as probe in superresolution microscopy. It is known that the photochromic reaction involves cis/trans isomerization of the chromophore and protonation/deprotonation of its phenol group, but the sequence in time of the two steps and their characteristic timescales are still the subject of much debate. We report here a comprehensive UV-visible transient absorption spectroscopy study of the photoactivation mechanism of Dronpa, covering all relevant timescales from ~100 fs to milliseconds. The Dronpa-2 variant was also studied and showed the same behavior. By carefully controlling the excitation energy to avoid multi-photon processes, we could measure both the spectrum and the anisotropy of the first photoactivation intermediate. We show that the observed few nanometer blue-shift of this intermediate is characteristic for a neutral cis chromophore, and that its anisotropy of ~0.2 is in good agreement with the reorientation of the transition dipole moment expected upon isomerization. These data constitute the first clear evidence that trans→cis isomerization of the chromophore precedes its deprotonation and occurs on the picosecond timescale, concomitantly to the excited-state decay. We found the deprotonation step to follow in ~10 μs and lead directly from the neutral cis intermediate to the final state.