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Article Dans Une Revue Nanoscale Research Letters Année : 2013

Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition

Résumé

We report the formation of a hybrid biological/artificial nanopore by the direct insertion of non-modified α-hemolysin at the entrance of a high aspect ratio (length/diameter) biomimetic nanopore. In this robust hybrid system, the protein exhibits the same polynucleotide discrimination properties as in the biological membrane and the polynucleotide dwell time is strongly increased. This nanopore is very promising for DNA sequencing applications where the high DNA translocation velocity and the fragility of the support are the main bottlenecks.
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Dates et versions

hal-01092673 , version 1 (09-12-2014)

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Simon Cabello-Aguilar, Sébastien Balme, Adib Abou Chaaya, Mikhael Bechelany, Emmanuel Balanzat, et al.. Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition. Nanoscale Research Letters, 2013, 5 (20), pp.5. ⟨10.1039/c3nr03683a⟩. ⟨hal-01092673⟩
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