The main thrust of this work was to improve the knowledge conerning soil biodiversity and related ecosystem services in soils from urban parks in several cities of the Région Centre, France. In this work, the pedological, geochemical and microbiological characteritics of surface soil were investigated in order to make an inventory of soil fertility in several urban parks of the major cities of the région Centre, France. The effects of agricultural practices on biomass, community structure and activity of micro-organisms were investigated in these soils in parallel with the determination of various pedo-physical and chemical parameters.