Super-resolution Microscopy Approaches for Live Cell Imaging
Résumé
By delivering optical images with spatial resolutions below the diffraction limit several super-resolution fluorescence microscopy techniques opened new opportunities to study biological structures with details approaching molecular structure sizes. They have now become methods of choice for imaging proteins and their nano-scale dynamic organizations in live cells. In this mini-review, we describe and compare the main far-field super-resolution approaches that allow studying endogenous or overexpressed proteins in live cells.
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