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Article Dans Une Revue Journal of Biological Chemistry Année : 2000

Multiple S-gene family members including natural antisense transcripts are differencially expressed during development of maize flowers

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Within the large Brassica S gene family, SLG (S locus glycoprotein) and SRK (S locus receptor kinase) participate to the control of pollen-stigma self-incompatibility. In the self-compatible species maize, S gene family members are predominantly expressed in vegetative organs but are also expressed to a lesser extent in the stigma (silk). To determine if the expression of any S gene family members correlates with female receptivity, we analyzed their expression in developing maize silks. We show that a large family of maize S transcripts is expressed in developing silks. Surprisingly, we isolated a cDNA complementary to a large portion of the antisense strand of the maize receptor kinase S domain. Rapid amplification of cDNA ends (RACE)-polymerase chain reaction, RNase protection, and Northern hybridization with single-stranded riboprobes confirmed that natural antisense S transcripts exist in leaves and seedling shoots and in all sexual tissues tested except mature pollen. These natural antisense S transcripts co-exist with several less abundant sense S transcripts. The accumulation of sense and antisense S transcripts is differentially regulated during pollen and silk development. Thus, these results support a role for S gene family members in sexual tissue development and/or compatible pollination and reveal a new level of complexity in the regulation and function of the S gene family in maize.
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hal-01017837 , version 1 (27-05-2021)

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Réjane Monod-Ansaldi, Annie Chaboud, Christian Dumas. Multiple S-gene family members including natural antisense transcripts are differencially expressed during development of maize flowers. Journal of Biological Chemistry, 2000, 275, pp.24146-24155. ⟨10.1074/jbc.M003047200⟩. ⟨hal-01017837⟩
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