The concentrations of 6 metals (Ag, Cd, Cu, Hg, Pb, and Zn) were investigated and compared in three tissues (arms, digestive gland and mantle) of three cephalopod species from the Tunisian waters: the common octopus (Octopus vulgaris), the common cuttlefish (Sepia officinalis) and the European squid (Loligo vulgaris). Whatever the species or the sites, the digestive gland displayed the highest concentrations of Ag, Cd, Cu, Pb and Zn highlighting its major role in their bioaccumulation and detoxification. This is also true for Hg but only for the digestive gland of O.vulgaris. Muscle from the arms and the mantle contained thus relatively low trace metal concentrations except for Hg in L.vulgaris and S.officinalis. Geographic comparison of metal concentrations in Tunisian cephalopods from 3 locations indicates that higher concentrations of Ag, Pb and Hg were observed in cephalopods from northern and eastern coasts, whereas the highest Cd levels was detected in the southeastern reflecting different conditions of exposure. Comparing the trace element concentrations between species, Ag, Cd, Cu, Hg and Zn concentrations were the highest in the digestive gland of octopuses. This may be related to the differences in ecological features and swimming behaviour among different cephalopod species. Effects of length and sex on metal levels were also considered, indicating a limited influence of sex on metal concentration.