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Communication Dans Un Congrès Année : 2011

The impact of bacterial diet in caenorhabditis elegans NMR metabolic profiling studies

Résumé

The nematode Caenorhabditis elegans became in the last years one of the most investigated animal models in the context of studies on aging. Among various approaches, NMR metabolomics investigations have emerged to characterize aging nematodes or long-lived mutant stains. Under standard conditions worms were grown on Normal Growth Medium (NGM) agar plates seeded with bacteria, which serve as food for the worms. After seeding the plates were in general used for several days to weeks. The aging of the bacteria on the plates implies a potential evolution in the quality of the nematode food, including viability of the bacteria and/or changes in their metabolite composition that may finally impact on the metabolism of C. elegans . Here, we present the impact of aging bacteria on the metabolic fingerprints of C. elegans using high-resolution magic angle spinning (HR-MAS) NMR as a whole organism profiling technique. The study was performed on C. elegans N2 wild type and dys-1(cx18) mutant strains. As nematode food source we investigated the Escherichia coli OP50 strain. O-PLS models built from 1H HR-MAS NMR data revealed a significant discrimination between worms grown on fresh or aged OP50 plates. The differences are mostly explained by an altered composition of the C. elegans lipid profile. While worms grown on aged NGM agar plates contain less monounsaturated fatty acids than those bred on plates freshly seeded with bacteria, they are shown to integrate exogenous cyclopropane fatty acids (CFA) in their lipid profiles. The CFA level can exceed 30% of the total amount of fatty acids in the worm, and come as direct intake from the bacterial food. Such findings are of primary importance in the general design of metabolomic studies on C. elegans, where biais or alteration in the lipid profiles, as influenced by the bacterial diet, should carefully be monitored.
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Dates et versions

hal-00980041 , version 1 (17-04-2014)

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  • HAL Id : hal-00980041 , version 1

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Andrei Bunescu, Kathrin Gieseler, Bénédicte Elena-Herrmann. The impact of bacterial diet in caenorhabditis elegans NMR metabolic profiling studies. 7th International Conference of the Metabolomics Society, Jun 2011, Cairns, Australia. ⟨hal-00980041⟩
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