Initial symbiont contact orchestrates host-organ-wide transcriptional changes that prime tissue colonization. - Archive ouverte HAL Access content directly
Journal Articles Cell Host and Microbe Year : 2013

Initial symbiont contact orchestrates host-organ-wide transcriptional changes that prime tissue colonization.

Eva E R Philipp
  • Function : Author
Marie-Christine Carpentier
Caitlin A Brennan
  • Function : Author
Lars Kraemer
  • Function : Author
Melissa A Altura
  • Function : Author
René Augustin
  • Function : Author
Robert Häsler
  • Function : Author
Elizabeth a C Heath-Heckman
  • Function : Author
Suzanne M Peyer
  • Function : Author
Julia Schwartzman
  • Function : Author
Bethany A Rader
  • Function : Author
Edward G Ruby
  • Function : Author
Margaret J Mcfall-Ngai
  • Function : Author

Abstract

Upon transit to colonization sites, bacteria often experience critical priming that prepares them for subsequent, specific interactions with the host; however, the underlying mechanisms are poorly described. During initiation of the symbiosis between the bacterium Vibrio fischeri and its squid host, which can be observed directly and in real time, approximately five V. fischeri cells aggregate along the mucociliary membranes of a superficial epithelium prior to entering host tissues. Here, we show that these few early host-associated symbionts specifically induce robust changes in host gene expression that are critical to subsequent colonization steps. This exquisitely sensitive response to the host's specific symbiotic partner includes the upregulation of a host endochitinase, whose activity hydrolyzes polymeric chitin in the mucus into chitobiose, thereby priming the symbiont and also producing a chemoattractant gradient that promotes V. fischeri migration into host tissues. Thus, the host responds transcriptionally upon initial symbiont contact, which facilitates subsequent colonization.

Dates and versions

hal-00972298 , version 1 (03-04-2014)

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Natacha Kremer, Eva E R Philipp, Marie-Christine Carpentier, Caitlin A Brennan, Lars Kraemer, et al.. Initial symbiont contact orchestrates host-organ-wide transcriptional changes that prime tissue colonization.. Cell Host and Microbe, 2013, 14 (2), pp.183-94. ⟨10.1016/j.chom.2013.07.006⟩. ⟨hal-00972298⟩
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