Development of microsatellite markers in Capsella Rubella and Capsella Bursa-Pastoris (Brassicaceae) 1 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue American Journal of Botany Année : 2011

Development of microsatellite markers in Capsella Rubella and Capsella Bursa-Pastoris (Brassicaceae) 1

Résumé

The Brassicaceae are an important family of about 338 genera distributed worldwide. It includes several species of crops, weeds, ornamentals, and the model organism Arabidopsis thaliana (L.) Heynh. Capsella bursa-pastoris (L.) Medik. And Capsella rubella Reut. belong to a small genus with only three species. Capsella species are closely related to the model organism Arabidopsis thaliana ( Couvreur et al., 2010 ). Capsella rubella is diploid (2 n = 2 x = 16). It grows around the Mediterranean Sea, and, more occasionally, in America and Australia where it was introduced ( Hurka and Neuffer, 1997 ). Capsella rubella has been described as a highly selfi ng plant ( Hurka et al., 1989 ) and has attracted attention as a model system for comparative genomic studies in crucifers ( Boivin et al., 2004 ). Complete genomic sequencing of C. rubella is under way, and sequences are currently available on the NCBI Trace archive http://www.ncbi.nlm.nih.gov/Traces/home/). Capsella bursa-pastoris (shepherd ' s purse) is tetraploid (2 n = 4 x = 32). It is distributed in ruderal habitats all over the world except in the tropics ( Hurka and Neuffer, 1997 ). Capsella bursa-pastoris is a predominantly selfi ng species with a fl exible mating system ( Hurka et al., 1989 ). Genetic variation in Capsella has been investigated using enzyme systems ( Hurka and Neuffer, 1997 ) and, more recently, gene sequencing ( Slotte et al., 2008 ). Genetic variation was found to be low, especially in C. rubella. To explore genetic variation using highly polymorphic markers, we developed 17 microsatellite loci.

Domaines

Botanique

Dates et versions

hal-00964329 , version 1 (24-03-2014)

Identifiants

Citer

Coraline C. Caullet, Fanny F. Pernin, Charles C. Poncet, Valérie V. Le Corre. Development of microsatellite markers in Capsella Rubella and Capsella Bursa-Pastoris (Brassicaceae) 1. American Journal of Botany, 2011, 98 (7), pp.e176-e179. ⟨10.3732/ajb.1100081⟩. ⟨hal-00964329⟩
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