SH-SAW sensing system combined with molecularly imprinted polymer (MIP) is introduced. The overall aim of this work is to develop and validate a quantitative, non-invasive diagnosis tool for specific detection of colon cancer biomarkers. This paper exhibits the validation of a MIP thin film coating process, allowing a high sensitivity of the resulting sensor. Morphology and print effect of the MIP film (sensitive layer) is the key of the sensor sensitivity to target biomarkers. Thereby, the real time detection measurement of the coated sensor under ethanol such as deep characterization of the film is presented. The rise of the MIP film thickness and detection of biomarkers analogs leads to a change of the sensors' responses to vapors. For example, 2126 ppm of ethanol generates frequency shifts of -1.4/-2.5kHz with MIP 200nm/600nm thickness. For the same ethanol concentration frequency shifts of -0.5/-1.3/-0.8 kHz, MIP/MIP after extraction/MIP after rebinding were observed respectively.