Role of Bordetella bronchiseptica adenylate cyclase in nasal colonization and in development of local and systemic immune responses in piglets - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Veterinary Research Année : 2005

Role of Bordetella bronchiseptica adenylate cyclase in nasal colonization and in development of local and systemic immune responses in piglets

Laurence Oyant
Dorothée Bourges
  • Fonction : Auteur
Claire Chevaleyre
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Dominique Raze
  • Fonction : Auteur
Camille Locht
  • Fonction : Auteur
Henri Salmon
  • Fonction : Auteur

Résumé

Two Bordetella bronchiseptica mutants, lacking the adenylate cyclase (Cya) or both Cya and pertactin (Prn), were compared with their parental strain NL1013 in their abilities to colonize the nose of neonate piglets and to induce local and systemic antibody responses against filamentous hemagglutinin (FHA) after intranasal (i.n.) inoculation. The number of bacteria recovered and the duration of infection in the nasal secretions were greater for the wild-type parent strain than for the Cya-deficient mutant, indicating that Cya plays an important role during B. bronchiseptica colonization of the nasal cavity. The double mutant did not colonize the nasal cavity and was less able to adhere to epithelial cells in vitro than the other two strains, supporting the hypothesis that Prn plays a major role in cell adhesion. In piglets inoculated with the wild type strain, anti-FHA IgM was found in the nasal secretions one week after inoculation, followed two weeks later by anti-FHA IgA; their presence was concomitant with decreases in bacterial counts. Anti-FHA IgG appeared at six weeks after infection in the serum. In contrast, i.n. inoculation with either mutant failed to induce a nasal secretory antibody response but did induce an earlier and higher IgM response in the serum than inoculation with the wild type strain. However, only the Cya-deficient mutant was able to prime the piglets for the development of a secondary nasal IgM and serum IgG response to FHA after intranasal inoculation with the wild type B. bronchiseptica.
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hal-00902959 , version 1 (11-05-2020)

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Laurence Oyant, Dorothée Bourges, Claire Chevaleyre, Dominique Raze, Camille Locht, et al.. Role of Bordetella bronchiseptica adenylate cyclase in nasal colonization and in development of local and systemic immune responses in piglets. Veterinary Research, 2005, 36 (1), pp.63-77. ⟨10.1051/vetres:2004056⟩. ⟨hal-00902959⟩
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