Analysis of gene mutation in plant cell wall by dielectric relaxation - Archive ouverte HAL Access content directly
Journal Articles Journal of Physics D: Applied Physics Year : 2012

Analysis of gene mutation in plant cell wall by dielectric relaxation

Abstract

Arabidopsis Thaliana is a plant composed mainly of cellulose and lignin. Geneticists need techniques able to make differences at the molecular level between modified plants (DML6, CAD C/D) and non-modified ones. Thermo-stimulated current (TSC) analysis is a promising route to identify gene mutations. For the non-modified plant, at low temperatures, TSC thermograms highlight three dielectric relaxation modes. From −150 to −110 ◦C, γCellulose is attributed to CH2OH and -OH groups of cellulose. Between −110 and −80 ◦C, βLignin is detected. From −80 to −40 ◦C, βCellulose is characteristic of the molecular mobility of glycosidic linkages. For the CAD C/D modified plants, only γCellulose and βLignin are observed; due to analogous enthalpy values, those modes have the same molecular origin as in the non-modified plant. So, the βLignin mode is associated with the molecular mobility of the lignin-OH groups. The CAD C/D gene mutation changes the chemical structure of lignin, which promotes hydrogen bonds in the network and inhibits molecular mobility of glucosidic rings. It is also interesting to note that the DML6 gene mutation induces a higher cooperativity of this βCellulose relaxation than in wild vegetal composites. In fact, this mutation promotes molecular mobility of glycosidic rings thanks to β1-4 glycosidic linkages.
Fichier principal
Vignette du fichier
Roig_8754.pdf (1.02 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-00880037 , version 1 (05-11-2013)

Identifiers

Cite

Frédéric Roig, Eric Dantras, Jacqueline Grima Pettenati, Colette Lacabanne. Analysis of gene mutation in plant cell wall by dielectric relaxation. Journal of Physics D: Applied Physics, 2012, vol. 45, pp. 1-7. ⟨10.1088/0022-3727/45/29/295402⟩. ⟨hal-00880037⟩
105 View
83 Download

Altmetric

Share

Gmail Facebook X LinkedIn More