Quantitative X-ray fluorescence analysis of heavy metals in natural contaminated plants
Résumé
Quantitative X-ray fluorescence measurements have been conducted on naturally lead-contaminated samples. The calibration procedure using the ratio of fluorescence to Compton scattered radiation was investigated using Monte Carlo simulation. Experimental results with low-energy photons (14 keV) and simulations show a very good linearity of the fluorescence to Compton ratio as a function of metal concentration. Lead (Pb), iron (Fe) and zinc (Zn) are measured in samples of "Phaseolus vulgaris" (bean seeds) which have been grown using a nutritive solution with different Pb doping. Natural contaminated samples are thus obtained. The calibration must be done for fixed conditions of X-ray energy and scattering angle, while X-ray beam intensity and detector to sample distance can change from one sample to another. Simulation allows to evaluate the matrix effect on the calibration curve, and shows that linearity is preserved even in the presence of other heavy elements in the fluorescence spectrum. However, calibration must be done using samples with similar matrix as it affects the slope of the curve.