Tomographic diffractive microscopy of transparent samples - Archive ouverte HAL
Article Dans Une Revue European Physical Journal: Applied Physics Année : 2008

Tomographic diffractive microscopy of transparent samples

Résumé

We report a tomographic diffractive microscope, which permits imaging non-labelled transparent or semi-transparent samples. Based on a combination of microholography with a tomographic illumination, our set-up creates 3-D images of the index of refraction distribution within the sample. One acquires successively interferograms, rotating the illumination (the specimen being static) and using phase-shifting holography. Within the first Born approximation, each interferogram is interpreted as a subset of the Fourier transform of the specimen index of refraction distribution. The reconstruction is therefore similar to synthetic aperture imaging: one recombines the information in the Fourier space, and a final Fourier transform gives a 3-D image of the specimen. First recalling the theoretical foundations, we then describe our experiment, and show initial results obtained on biological samples.
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Dates et versions

hal-00869566 , version 1 (02-12-2013)

Identifiants

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Bertrand Simon, Matthieu Debailleul, Vincent Georges, Vincent Lauer, Olivier Haeberlé. Tomographic diffractive microscopy of transparent samples. European Physical Journal: Applied Physics, 2008, 44, pp.29-35. ⟨10.1051/epjap:2008049⟩. ⟨hal-00869566⟩
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