Biotin-end-functionalized highly fluorescent water-soluble polymers - Archive ouverte HAL
Article Dans Une Revue Polymer Chemistry Année : 2013

Biotin-end-functionalized highly fluorescent water-soluble polymers

Résumé

Many biosensing and imaging systems use fluorescence detection. We present the synthesis of biotin-end-functionalized highly fluorescent water-soluble polymers for potential use in biotin-avidin systems. Statistical polymers of N-acryloylmorpholine (NAM) and N-acryloxysuccinimide (NAS) were prepared by RAFT polymerization using a biotinylated chain transfer agent that ensured 95% end-functionalization of the chains. They were further labeled with a lucifer yellow (LY) dye, yielding 7 to 62 LY fluorophores per polymer chain. The resulting polymers exhibited reduced fluorescence self-quenching, with 7- to 43-fold higher brightness than free LY dye. In addition, they featured low pH sensitivity and very good photobleaching resistance. Moreover, we showed that a more extended polymer conformation was beneficial to the binding of the terminal biotin with streptavidin. This work paves the way for the development of polymers for signal amplification in biosensing assays, labeling of biotin-receptors at cell surfaces in some cancer studies, labeling of antibodies and microscopy imaging purposes.
Fichier non déposé

Dates et versions

hal-00867740 , version 1 (30-09-2013)

Identifiants

Citer

Paula Relogio, Maël Bathfield, Zofia Haftek-Terreau, Mariana Beija, Arnaud Favier, et al.. Biotin-end-functionalized highly fluorescent water-soluble polymers. Polymer Chemistry, 2013, 4, pp.2968-2981. ⟨10.1039/c3py00059a⟩. ⟨hal-00867740⟩
203 Consultations
0 Téléchargements

Altmetric

Partager

More