Antagonism versus cooperativity with TALE cofactors at the base of the functional diversification of Hox protein function - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS Genetics Année : 2013

Antagonism versus cooperativity with TALE cofactors at the base of the functional diversification of Hox protein function

Résumé

Extradenticle (Exd) and Homothorax (Hth) function as positive transcriptional cofactors of Hox proteins, helping them to bind specifically their direct targets. The posterior Hox protein Abdominal-B (Abd-B) does not require Exd/Hth to bind DNA; and, during embryogenesis, Abd-B represses hth and exd transcription. Here we show that this repression is necessary for Abd-B function, as maintained Exd/Hth expression results in transformations similar to those observed in loss-of-function Abd-B mutants. We characterize the cis regulatory module directly regulated by Abd-B in the empty spiracles gene and show that the Exd/Hth complex interferes with Abd-B binding to this enhancer. Our results suggest that this novel Exd/Hth function does not require the complex to bind DNA and may be mediated by direct Exd/Hth binding to the Abd-B homeodomain. Thus, in some instances, the main positive cofactor complex for anterior Hox proteins can act as a negative factor for the posterior Hox protein Abd-B. This antagonistic interaction uncovers an alternative way in which MEIS and PBC cofactors can modulate Abd-B like posterior Hox genes during development.
Fichier principal
Vignette du fichier
journal.pgen.1003252.PDF (1.69 Mo) Télécharger le fichier
Origine Publication financée par une institution
Loading...

Dates et versions

hal-00862286 , version 1 (19-09-2018)

Licence

Identifiants

Citer

María Luisa Rivas, Jose Manuel Espinosa-Vázquez, Nagraj Sambrani, Stephen Greig, Samir Merabet, et al.. Antagonism versus cooperativity with TALE cofactors at the base of the functional diversification of Hox protein function. PLoS Genetics, 2013, 9 (2), pp.e1003252. ⟨10.1371/journal.pgen.1003252⟩. ⟨hal-00862286⟩
53 Consultations
31 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More