In vivo imaging of human retinal microvasculature using adaptive optics scanning light ophthalmoscope fluorescein angiography. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biomedical optics express Année : 2013

In vivo imaging of human retinal microvasculature using adaptive optics scanning light ophthalmoscope fluorescein angiography.

Alexander Pinhas
  • Fonction : Auteur
Nishit Shah
  • Fonction : Auteur
Toco y Chui
  • Fonction : Auteur
Drew Scoles
  • Fonction : Auteur
Yusufu N Sulai
  • Fonction : Auteur
Rishard Weitz
  • Fonction : Auteur
Joseph B Walsh
  • Fonction : Auteur
Joseph Carroll
  • Fonction : Auteur
Alfredo Dubra
  • Fonction : Auteur
Richard B Rosen
  • Fonction : Auteur

Résumé

The adaptive optics scanning light ophthalmoscope (AOSLO) allows visualization of microscopic structures of the human retina in vivo. In this work, we demonstrate its application in combination with oral and intravenous (IV) fluorescein angiography (FA) to the in vivo visualization of the human retinal microvasculature. Ten healthy subjects ages 20 to 38 years were imaged using oral (7 and/or 20 mg/kg) and/or IV (500 mg) fluorescein. In agreement with current literature, there were no adverse effects among the patients receiving oral fluorescein while one patient receiving IV fluorescein experienced some nausea and heaving. We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure. As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses. The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes.

Dates et versions

hal-00859908 , version 1 (09-09-2013)

Identifiants

Citer

Alexander Pinhas, Michael Dubow, Nishit Shah, Toco y Chui, Drew Scoles, et al.. In vivo imaging of human retinal microvasculature using adaptive optics scanning light ophthalmoscope fluorescein angiography.. Biomedical optics express, 2013, 4 (8), pp.1305-17. ⟨10.1364/BOE.4.001305⟩. ⟨hal-00859908⟩
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