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Article Dans Une Revue PLoS ONE Année : 2011

T cells recognizing a peptide contaminant undetectable by mass spectrometry

Résumé

Synthetic peptides are widely used in immunological research as epitopes to stimulate their cognate T cells. These preparations are never completely pure, but trace contaminants are commonly revealed by mass spectrometry quality controls. In an effort to characterize novel major histocompatibility complex (MHC) Class I-restricted β-cell epitopes in non-obese diabetic (NOD) mice, we identified islet-infiltrating CD8+ T cells recognizing a contaminating peptide. The amount of this contaminant was so small to be undetectable by direct mass spectrometry. Only after concentration by liquid chromatography, we observed a mass peak corresponding to an immunodominant islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP)$_{206-214}$ epitope described in the literature. Generation of CD8+ T-cell clones recognizing IGRP$_{206-214}$) using a novel method confirmed the identity of the contaminant, further underlining the immunodominance of IGRP$_{206-214}$. If left undetected, minute impurities in synthetic peptide preparations may thus give spurious results.
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hal-00858150 , version 1 (23-03-2022)

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Vedran Brezar, Slobodan Culina, Thomas Østerbye, François Guillonneau, Giovanni Chiappetta, et al.. T cells recognizing a peptide contaminant undetectable by mass spectrometry. PLoS ONE, 2011, 6 (12), pp.e28866. ⟨10.1371/journal.pone.0028866⟩. ⟨hal-00858150⟩
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