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Article Dans Une Revue Plant Physiology and Biochemistry Année : 2010

Assessment of mitochondria as a compartment for phosphatidylinositol synthesis in Solanum tuberosum.

Jacques Davy de Virville
  • Fonction : Auteur
Françoise Cochet
  • Fonction : Auteur
Marie-Noëlle Soler
  • Fonction : Auteur
Michèle Hoffelt
  • Fonction : Auteur
Eric Ruelland
Alain Zachowski
  • Fonction : Auteur
Sylvie Collin

Résumé

The outer mitochondrial membrane is particularly rich in phosphatidylinositol (PtdIns), a phospholipid found in different amounts in all eukaryotic membranes, but not synthesized in situ by all. PtdIns is therefore subjected to traffic from the synthesizing membranes to the non-synthesizing ones. The contribution of mitochondria to the cell PtdIns pool has never been the focus of a specific study in plants, whereas in yeast, the presence of the enzyme responsible for synthesis, PtdIns synthase (PIS, cytidine 5'-diphospho-1,2-diacyl-sn-glycerol:myo-inositol 3-phosphatidyltransferase, EC 2.7.8.11), has clearly been demonstrated in mitochondria. As these organelles have now been shown to be responsible for the synthesis of several lipids, the present work aimed at evaluating mitochondria as a compartment for the synthesis of PtdIns in plants. The sub-cellular localization of PIS was studied in Solanum tuberosum L. by membrane fractionation, enzymatic analysis and by confocal microscopy in living cells. In potato, beside the endoplasmic reticulum, the activity of PIS was found to be tightly associated to mitochondria. Using a fluorescent reporter fusion, the enzyme was also found to be associated to these organelles. The enzyme was not present at the plasma membrane. A comparison of the localization in other cell systems suggests that the mitochondrial localization could be regulated.

Dates et versions

hal-00855953 , version 1 (30-08-2013)

Identifiants

Citer

Jacques Davy de Virville, Spencer Brown, Françoise Cochet, Marie-Noëlle Soler, Michèle Hoffelt, et al.. Assessment of mitochondria as a compartment for phosphatidylinositol synthesis in Solanum tuberosum.. Plant Physiology and Biochemistry, 2010, 48 (12), pp.952-60. ⟨10.1016/j.plaphy.2010.09.004⟩. ⟨hal-00855953⟩
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