Cardosin A contains two vacuolar sorting signals using different vacuolar routes in tobacco epidermal cells. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Plant Journal Année : 2013

Cardosin A contains two vacuolar sorting signals using different vacuolar routes in tobacco epidermal cells.

Cláudia Pereira
  • Fonction : Auteur
Susana Pereira
  • Fonction : Auteur
Béatrice Satiat-Jeunemaitre
  • Fonction : Auteur
  • PersonId : 750006
  • IdHAL : bsj
José Pissarra
  • Fonction : Auteur

Résumé

: Several vacuolar sorting determinants (VSDs) have been described for protein trafficking to the vacuoles in plant cells. Because of the variety in plant models, cell types and experimental approaches used to decipher vacuolar targeting processes, it is not clear whether the three well-known groups of VSDs identified so far exhaust all the targeting mechanisms, nor if they reflect certain protein types or families. The vacuolar targeting mechanisms of the aspartic proteinases family, for instance, are not yet fully understood. In previous studies, cardosin A has proven to be a good reporter for studying the vacuolar sorting of aspartic proteinases. We therefore propose to explore the roles of two different cardosin A domains, common to several aspartic proteinases [i.e. the plant-specific insert (PSI) and the C-terminal peptide VGFAEAA] in vacuolar sorting. Several truncated versions of the protein conjugated with fluorescent protein were made, with and without these putative sorting determinants. These domains were also tested independently, for their ability to sort other proteins, rather than cardosin A, to the vacuole. Fluorescent chimaeras were tracked in vivo, by confocal laser scanning microscopy, in Nicotiana tabacum cells. Results demonstrate that either the PSI or the C terminal was necessary and sufficient to direct fluorescent proteins to the vacuole, confirming that they are indeed vacuolar sorting determinants. Further analysis using blockage experiments of the secretory pathway revealed that these two VSDs mediate two different trafficking pathways.
Fichier non déposé

Dates et versions

hal-00855561 , version 1 (29-08-2013)

Identifiants

Citer

Cláudia Pereira, Susana Pereira, Béatrice Satiat-Jeunemaitre, José Pissarra. Cardosin A contains two vacuolar sorting signals using different vacuolar routes in tobacco epidermal cells.. Plant Journal, 2013, epub ahead of print. ⟨10.1111/tpj.12274⟩. ⟨hal-00855561⟩
32 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More