Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular and Cellular Proteomics Année : 2012

Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.

Willy V Bienvenut
David Sumpton
  • Fonction : Auteur
Sergio Lilla
  • Fonction : Auteur
Thierry Meinnel
Carmela Giglione

Résumé

N-terminal modifications play a major role in the fate of proteins in terms of activity, stability, or subcellular compartmentalization. Such modifications remain poorly described and badly characterized in proteomic studies, and only a few comparison studies among organisms have been made available so far. Recent advances in the field now allow the enrichment and selection of N-terminal peptides in the course of proteome-wide mass spectrometry analyses. These targeted approaches unravel as a result the extent and nature of the protein N-terminal modifications. Here, we aimed at studying such modifications in the model plant Arabidopsis thaliana to compare these results with those obtained from a human sample analyzed in parallel. We applied large scale analysis to compile robust conclusions on both data sets. Our data show strong convergence of the characterized modifications especially for protein N-terminal methionine excision, co-translational N-α-acetylation, or N-myristoylation between animal and plant kingdoms. Because of the convergence of both the substrates and the N-α-acetylation machinery, it was possible to identify the N-acetyltransferases involved in such modifications for a small number of model plants. Finally, a high proportion of nuclear-encoded chloroplast proteins feature post-translational N-α-acetylation of the mature protein after removal of the transit peptide. Unlike animals, plants feature in a dedicated pathway for post-translational acetylation of organelle-targeted proteins. The corresponding machinery is yet to be discovered.

Dates et versions

hal-00851370 , version 1 (13-08-2013)

Identifiants

Citer

Willy V Bienvenut, David Sumpton, Aude Martinez, Sergio Lilla, Christelle Espagne, et al.. Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular and Cellular Proteomics, 2012, 11 (6), pp.M111.015131. ⟨10.1074/mcp.M111.015131⟩. ⟨hal-00851370⟩
32 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More