Osmotic stress and phosphate limitation alter production of cell-to-cell signal molecules and rhamnolipid biosurfactant by Pseudomonas aeruginosa. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Letters Année : 2005

Osmotic stress and phosphate limitation alter production of cell-to-cell signal molecules and rhamnolipid biosurfactant by Pseudomonas aeruginosa.

Résumé

In Pseudomonas aeruginosa, rhamnolipid production is controlled by the quorum-sensing system RhlRI, which itself depends on LasRI. These systems use cell-to-cell signal molecules: N-butyryl-l-homoserine lactone (C4-HSL) and N-(3-oxododecanoyl)-l-homoserine lactone (3OC(12)-HSL), respectively. Whereas both HSLs were produced in M63 medium, rhamnolipid synthesis was not achieved. Phosphate limitation reduced the HSL concentrations, while allowing rhamnolipid production. Hyperosmotic shock applied during the exponential growth phase stopped the accumulation of 3OC(12)-HSL, and prevented C4-HSL and rhamnolipid production. These defects result from lower expression of genes involved in C4-HSL and rhamnolipid syntheses. The osmoprotectant glycine betaine partially restored C4-HSL and rhamnolipid production.

Domaines

Bactériologie

Dates et versions

hal-00846228 , version 1 (18-07-2013)

Identifiants

Citer

Alexis Bazire, Alexandra Dheilly, Farès Diab, Danièle Morin, Mohamed Jebbar, et al.. Osmotic stress and phosphate limitation alter production of cell-to-cell signal molecules and rhamnolipid biosurfactant by Pseudomonas aeruginosa.. FEMS Microbiology Letters, 2005, 253 (1), pp.125-31. ⟨10.1016/j.femsle.2005.09.029⟩. ⟨hal-00846228⟩
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