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Article Dans Une Revue PLoS ONE Année : 2013

Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility.

Résumé

Basophils co-express Fc epsilon RI alpha and CD49b, the alpha-2 chain of integrin-type receptor VLA-2 (alpha 2 beta 1), which recognizes type-1 collagen as a major natural ligand. The physiological relevance of this integrin for interactions with extracellular bone marrow matrix remains unknown. Herein, we examined the expression of several receptors of this family by bone marrow-derived basophils sorted either ex-vivo or after culture with IL-3. Having established that both populations display CD49d, CD49e and CD49f (alpha-4, alpha-5 and alpha-6 integrins subunits, respectively), we addressed receptor functions by measuring migration, adhesion, proliferation and survival after interacting with matched natural ligands. Type I collagen, laminin and fibronectin promoted basophil migration/adhesion, the former being the most effective. None of these ligands affected basophil viability and expansion. Interactions between basophils and extracellular matrix are likely to play a role in situ, as supported by confocal 3D cell imaging of femoral bone marrow sections, which revealed basophils exclusively in type-1 collagen-enriched niches that contained likewise laminin and fibronectin. This is the first evidence for a structure/function relationship between basophils and extracellular matrix proteins inside the mouse bone marrow.

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Autre

Dates et versions

hal-00845010 , version 1 (16-07-2013)

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S. Smaniotto, E. Schneider, N. Goudin, R. Bricard-Rignault, F. Machavoine, et al.. Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility.. PLoS ONE, 2013, ?, pp.?-?. ⟨10.1371/journal.pone.0070292⟩. ⟨hal-00845010⟩

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