Detecting unknown sequences with DNA microarrays: explorative probe design strategies. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Environmental Microbiology Année : 2012

Detecting unknown sequences with DNA microarrays: explorative probe design strategies.

Résumé

Designing environmental DNA microarrays that can be used to survey the extreme diversity of microorganisms existing in nature, represents a stimulating challenge in the field of molecular ecology. Indeed, recent efforts in metagenomics have produced a substantial amount of sequence information from various ecosystems, and will continue to accumulate large amounts of sequence data given the qualitative and quantitative improvements in the next-generation sequencing methods. It is now possible to take advantage of these data to develop comprehensive microarrays by using explorative probe design strategies. Such strategies anticipate genetic variations and thus are able to detect known and unknown sequences in environmental samples. In this review, we provide a detailed overview of the probe design strategies currently available to construct both phylogenetic and functional DNA microarrays, with emphasis on those permitting the selection of such explorative probes. Furthermore, exploration of complex environments requires particular attention on probe sensitivity and specificity criteria. Finally, these innovative probe design approaches require exploiting newly available high-density microarray formats.
Fichier principal
Vignette du fichier
Dugat-Bony2012_Detecting unknown sequences with DNA microarrays_1.pdf (410.85 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-00822134 , version 1 (28-05-2020)

Identifiants

Citer

Eric Dugat-Bony, Eric Peyretaillade, Nicolas Parisot, Corinne Biderre-Petit, Faouzi Jaziri, et al.. Detecting unknown sequences with DNA microarrays: explorative probe design strategies.. Environmental Microbiology, 2012, 14 (2), pp.356-71. ⟨10.1111/j.1462-2920.2011.02559.x⟩. ⟨hal-00822134⟩
122 Consultations
85 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More