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Article Dans Une Revue Molecular Imaging and Biology Année : 2013

Synchrotron Radiation X-Ray Phase Micro-computed Tomography as a New Method to Detect Iron Oxide Nanoparticles in the Brain

M. Langer
Cécile Olivier
T. H. Cho
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F. Peyrin

Résumé

PurposeThe purpose of this study was to introduce synchrotron radiation X-ray phase computed tomography (SR-PCT) as a new method of visualizing ultrasmall superparamagnetic particles of iron oxide (USPIO) distribution into the brains of mice with neuroinflammation.ProceduresThe sensitivity of the technique was assessed by performing back-to-back SR-PCT and magnetic resonance imaging (MRI) in mice stereotaxically injected with a range of USPIO concentrations. Eight mice with cerebral ischemia were then intravenously injected with USPIOs and imaged back-to-back with MRI and SR-PCT.ResultsSR-PCT proved sensitive enough to detect iron in nanomolar quantities. In stroke-induced animals, SR-PCT showed hyperintense areas in the regions of MR signal loss and immunostaining for macrophages. SR-PCT, moreover, identified brain anatomy as clearly as histology, without the need for sectioning or staining, with an examination time of 44 min per brain at an isotropic spatial resolution of 8 μm.ConclusionSR-PCT has potential for cellular imaging in intact brain, with unequaled neuroanatomy.

Dates et versions

hal-00821105 , version 1 (07-05-2013)

Identifiants

Citer

M. Marinescu, M. Langer, A. Durand, Cécile Olivier, A. Chabrol, et al.. Synchrotron Radiation X-Ray Phase Micro-computed Tomography as a New Method to Detect Iron Oxide Nanoparticles in the Brain. Molecular Imaging and Biology, 2013, 15 (5), pp.552-559. ⟨10.1007/s11307-013-0639-6⟩. ⟨hal-00821105⟩
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