Processing of asparagine-linked oligosaccharides is an early biochemical marker of the enterocytic differentiation of HT-29 cells. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Cellular Biochemistry Année : 1989

Processing of asparagine-linked oligosaccharides is an early biochemical marker of the enterocytic differentiation of HT-29 cells.

E. Ogier-Denis
  • Fonction : Auteur
C. Bauvy
  • Fonction : Auteur
M. Aubery
  • Fonction : Auteur
P. Codogno
  • Fonction : Auteur
Christian Sapin
Monique Rousset
  • Fonction : Auteur
A. Zweibaum
  • Fonction : Auteur

Résumé

The inability of HT-29 cells to undergo an enterocytic differentiation when grown in a glucose-containing (Glc+) medium has been recently correlated to an overall impairment of N-glycan processing. These results were obtained using confluent HT-29 cells in which the differentiation characteristics are fully expressed under differentiation permissive conditions (glucose-deprived medium, Glc-). Whether these changes of N-glycan processing appear progressively during the cell growth or are already present from the beginning of the culture was investigated in this work by comparing the actual status of N-glycan processing in both exponentially growing Glc+ and Glc- HT-29 cells. Under these conditions, HT-29 cells do not express any characteristics of enterocytic differentiation, even when grown in differentiation permissive conditions. We show here that the conversion of high-mannose to complex glycoproteins is, however, severely reduced in HT-29 cells grown in differentiation non-permissive conditions (HT-29 Glc+) whatever the phase of growth studied. In contrast, HT-29 cells grown in differentiation permissive conditions (HT-29 Glc-) display a normal pattern of N-glycan processing in both the exponential and the stationary phase of growth. We also show that both growing and confluent HT-29 Glc+ cells accumulate Man GlcNAc2 species, thus suggesting that there is an important regulatory point at this level. We therefore conclude that the N-glycan processing may be used as an early biochemical probe for the enterocytic differentiation of HT-29 cells. Whether these early changes result from an early metabolic regulation or are the consequence of a genetic control remains to be studied.

Domaines

Chimie organique

Dates et versions

hal-00814595 , version 1 (17-04-2013)

Identifiants

Citer

E. Ogier-Denis, C. Bauvy, M. Aubery, P. Codogno, Christian Sapin, et al.. Processing of asparagine-linked oligosaccharides is an early biochemical marker of the enterocytic differentiation of HT-29 cells.. Journal of Cellular Biochemistry, 1989, 41 ((1)), pp.13-23. ⟨10.1002/jcb.240410103⟩. ⟨hal-00814595⟩
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