Microplanktonic Community Structure in a Coastal System Relative to a Phaeocystis Bloom Inferred from Morphological and Tag Pyrosequencing Methods - Archive ouverte HAL Access content directly
Journal Articles PLoS ONE Year : 2012

Microplanktonic Community Structure in a Coastal System Relative to a Phaeocystis Bloom Inferred from Morphological and Tag Pyrosequencing Methods

Dionigia Meloni
  • Function : Author
Giovanna Sanciu
  • Function : Author
Magali Chabé
Laurence Delhaes
  • Function : Author
Eric Viscogliosi
Télesphore Sime-Ngando

Abstract

Background Massive phytoplankton blooms, like the recurrent Phaeocystis proliferation observed every year in the Eastern English Channel (EEC), have a significant influence on the overall planktonic community structure and their food web dynamics. As well as being an important area for local fisheries, the EEC is an ideal ecosystem for work on microbial diversity. This is because, although its environmental context is relatively complex, it is reasonably well understood due to several years of monitoring and morphological observations of its planktonic organisms. The objective of our study was to better understand the under-explored microbial eukaryotic diversity relative to the Phaeocystis bloom. Methodology and Principal Findings The community structure of microplankton (diatoms, haptophytes, ciliates and dinoflagellates) was studied through morphological observations and tag pyrosequencing. During the annual Phaeocystis spring bloom, the phytoplankton biomass increased by 34-fold, while the microzooplankton biomass showed a 4-fold increase, representing on average about 4.6% of the biomass of their phytoplankton prey. Tag pyrosequencing unveiled an extensive diversity of Gymnodiniaceae, with G. spirale and G. fusiformis representing the most abundant reads. An extended diversity of Phaeocystales, with partial 18S rDNA genes sequence identity as low as 85% was found, with taxa corresponding to P. globosa, but also to unknown Phaeocystaceae

Domains

Oceanography

Dates and versions

hal-00806968 , version 1 (02-04-2013)

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Sébastien Monchy, Jean-David Grattepanche, Elsa Breton, Dionigia Meloni, Giovanna Sanciu, et al.. Microplanktonic Community Structure in a Coastal System Relative to a Phaeocystis Bloom Inferred from Morphological and Tag Pyrosequencing Methods. PLoS ONE, 2012, e39924, pp.7. ⟨10.1371/journal.pone.0039924⟩. ⟨hal-00806968⟩
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