Pre- and post-treatment with cyclosporine A in a rat model of transient focal cerebral ischaemia with multimodal MRI screening - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Stroke Année : 2013

Pre- and post-treatment with cyclosporine A in a rat model of transient focal cerebral ischaemia with multimodal MRI screening

Résumé

Background Irreversible damage may occur at reperfusion after sustained cerebral ischaemia. Aims We investigated the value of cyclosporine A for reducing the infarct size in a model of transient middle cerebral artery occlusion. Methods Twenty-seven Sprague-Dawley rats sustained a middle cerebral artery occlusion of one-hour. Acute multimodal Magnetic Resonance Imaging (MRI) was used during occlusion to confirm the success of surgery and measure baseline lesion size. Animals were randomly treated by: (i) intracarotid cyclosporine A (10 mg/kg) 20 mins before middle cerebral artery occlusion (pretreatment group); (ii) intracarotid cyclosporine A (10 mg/kg) immediately after reperfusion (post-treatment group); and (iii) intracarotid saline immediately after reperfusion. Results Histopathological measurements on day 1 showed a significant reduction of infarct size in the pretreatment group compared to the post-treatment (percentage values of ipsilateral hemispheres: 16 ± 5% vs. 29 ± 11%, P = 0*004) and saline groups (16 ± 5% vs. 42 ± 12%, P = 0*015). No significant difference was observed between the post-treatment and saline groups (P = 0*065). Behavioural examinations on day 1 showed no significant difference between groups. Immunohistochemistry showed a statistically significant reduction of microglial cell count in the pretreatment group compared to either saline or cyclosporine A post-treatment groups. Conclusions We conclude that intracarotid cyclosporine A is effective in reducing infarct size when given prior to ischaemia, but not when administered at reperfusion.
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Dates et versions

hal-00798015 , version 1 (07-03-2013)

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Tae-Hee Cho, Pierre Aguettaz, Oscar Campuzano, Christiane Charriaut-Marlangue, Adrien Riou, et al.. Pre- and post-treatment with cyclosporine A in a rat model of transient focal cerebral ischaemia with multimodal MRI screening. International Journal of Stroke, 2013, 8 (8), pp.669-674. ⟨10.1111/j.1747-4949.2012.00849.x⟩. ⟨hal-00798015⟩
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