Human hepatic cell behavior on polysulfone membrane with double porosity level - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Membrane Science Année : 2013

Human hepatic cell behavior on polysulfone membrane with double porosity level

Résumé

In the membrane-based bioartificial livers developed up to now,the hepatic cells were located either in the fibers lumen or in the cartridge,with limited capacity for cell hosting.Here,we designed a polysulfone (PSU) membrane with a double porosity level:(i)surface macroporosity emerging in macrochambers accessible to hepatic cell colonization;(ii)microporosity to ensure gas and molecule transfers between macrochambers and supernatant,as well as potential immune barrier. ESEM and X-ray tomography confirmed that macrochambers accessed the membrane surface and were inter-connected. Biocompatibility and performances of this PSU membrane with double porosity level were compared to classical semi-permeable structures,following cell organization,cell proliferation and liver specific activities over a 9 days incubation. Macrochambers were colonized by hepatic cells, leading to higher albumin synthesis compared to control.Therefore,this membrane with double porosity appeared as a promising support to offer an inner 3D environment adequate to cell proliferation to form a liver-like tissue.

Domaines

Génie chimique
Fichier principal
Vignette du fichier
Bacchin_8286.pdf (628.2 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-00788387 , version 1 (14-02-2013)

Identifiants

Citer

Murielle Dufresne, Patrice Bacchin, Giulia Cerino, Jean-Christophe Remigy, Gerard Nathanael Adrianus, et al.. Human hepatic cell behavior on polysulfone membrane with double porosity level. Journal of Membrane Science, 2013, vol. 428, pp. 454-461. ⟨10.1016/j.memsci.2012.10.041⟩. ⟨hal-00788387⟩
92 Consultations
212 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More