The overall objective of this work is to develop and validate a quantitative, non-invasive therapeutic tool to detect selected urinary modified nucleosides as biomarkers of colorectal cancer chemotherapy and to monitor in fine the efficiency of the chemotherapy. Our methodology takes the advantage of high sensitivity of acoustic biosensor combined with high selectivity and robustness of thin molecularly imprinted polymer (MIP) film. In this paper we present a process based on a thin film of a MIP of adenosine 5'-monophosphate (AMP) coating on the sensor surface which is compatible with the acoustic wave propagation. Detection tests of AMP have been performed in aqueous media. The sensor response was recorded in terms of synchronous frequency and total insertion losses after both steps: extraction from, rebinding by the MIP layer. A frequency decrease of 6.875 Hz was recorded for 25μg/mL AMP concentration.