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Article Dans Une Revue Human Immunology Année : 2012

Analyzing HLA-G polymorphisms in children from women with scleroderma

Résumé

Embryos during pregnancy and organs during transplantation, express high levels of soluble HLA-G (sHLA-G) molecules for successful implantation and protection against maternal immune cells or recipient's cells. We and others have shown that women with scleroderma (SSc) carry cells/DNA arising from pregnancy, so-called fetal microchimerism (Mc) more often and in higher quantities than healthy women decades after delivery. We hypothesized that high levels of fetal Mc were the consequence of a fetus with a high sHLA-G profile, therefore that children from women with SSc would have this profile more often than children from healthy women. High sHLA-G secretor profile is influenced by at least two variations in the HLA-G 3' untranslated region (UTR): a 14bp deletion in exon 8 and the presence of cysteine (C) in position +3142 and by one variation in the 5' Upstream Regulatory Region (URR) at position -725. By a previously developed three-step multiplex PCR SNaPshot method, we evaluated 16 HLA-G polymorphisms in DNA samples from the first-born children of 39 women with SSc and 32 healthy women. Contrary to expectations, children from women with SSc did not have a high sHLA-G profile, but rather the opposite. We discuss possible reasons for this result and future orientations for HLA-G studies in SSc.

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Dates et versions

hal-00763261 , version 1 (10-12-2012)

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Christophe Picard, Julie Di Cristofaro, Doua F. Azzouzb, Sami B. Kanaanb, Jean Roudier, et al.. Analyzing HLA-G polymorphisms in children from women with scleroderma. Human Immunology, 2012, ⟨10.1016/j.humimm.2012.11.030⟩. ⟨hal-00763261⟩
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