Comparison of extractable DNA from bone following six-month exposure to outdoor conditions, garden loam, mold contamination or room storage. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Medicine, Science and the Law Année : 2012

Comparison of extractable DNA from bone following six-month exposure to outdoor conditions, garden loam, mold contamination or room storage.

Résumé

Femur bone sections from a single donor were exposed for six months to (i) outdoor conditions (exposure to sun, rain, etc.); (ii) water-vapour saturated environment favourable to mould proliferation and (iii) humic-garden soil. Following these treatments, DNA was extracted and yields were compared with that of a control bone fragment kept under optimal laboratory storage conditions. Our results demonstrate that both mould and soil are very detrimental to bone DNA conservation since more than 97% of the bone DNA was lost in these samples as compared with the control condition. Outdoor exposure gives an intermediate result with 30% of the DNA still present in the bone. Thus, environments favourable to microorganisms proliferation appear detrimental to bone DNA conservation and are a bad prognostic should bone remains be used for genetic identification purpose. Comparatively, open-air exposure is much more favourable to bone DNA analysis.
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Dates et versions

hal-00761158 , version 1 (05-12-2012)

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Loïc Startari, Jean-Noël Benoit, Gérald Quatrehomme, Georges Carle, Philippe Pognonec. Comparison of extractable DNA from bone following six-month exposure to outdoor conditions, garden loam, mold contamination or room storage.. Medicine, Science and the Law, 2012, epub ahead of print. ⟨10.1258/msl.2012.012018⟩. ⟨hal-00761158⟩
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