A universal nucleoside with strong two-band switchable fluorescence and sensitivity to the environment for investigating DNA interactions. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of the American Chemical Society Année : 2012

A universal nucleoside with strong two-band switchable fluorescence and sensitivity to the environment for investigating DNA interactions.

Résumé

With the aim of developing a new tool to investigate DNA interactions, a nucleoside analogue incorporating a 3-hydroxychromone (3HC) fluorophore as a nucleobase mimic was synthesized and incorporated into oligonucleotide chains. In comparison with existing fluorescent nucleoside analogues, this dye features exceptional environmental sensitivity switching between two well-resolved fluorescence bands. In labeled DNA, this nucleoside analogue does not alter the duplex conformation and exhibits a high fluorescence quantum yield. This probe is up to 50-fold brighter than 2-aminopurine, the fluorescent nucleoside standard. Moreover, the dual emission is highly sensitive to the polarity of the environment; thus, a strong shielding effect of the flanking bases from water was observed. With this nucleoside, the effect of a viral chaperone protein on DNA base stacking was site-selectively monitored.

Domaines

Chimie organique
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Dates et versions

hal-00758299 , version 1 (28-11-2012)

Identifiants

Citer

Dmytro Dziuba, Viktoriia y Postupalenko, Marie Spadafora, Andrey S Klymchenko, Vincent Guérineau, et al.. A universal nucleoside with strong two-band switchable fluorescence and sensitivity to the environment for investigating DNA interactions.. Journal of the American Chemical Society, 2012, 134 (24), pp.10209-13. ⟨10.1021/ja3030388⟩. ⟨hal-00758299⟩
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