A PQL (protein quantity loci) analysis of mature pea seed proteins identifies loci determining seed protein composition. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proteomics Année : 2011

A PQL (protein quantity loci) analysis of mature pea seed proteins identifies loci determining seed protein composition.

Michael Bourgeois
  • Fonction : Auteur
Françoise Jacquin
  • Fonction : Auteur
Florence Cassecuelle
  • Fonction : Auteur
Vincent Savois
  • Fonction : Auteur
Laurence Quillien
  • Fonction : Auteur
Myriam Huart
Pascal Marget
  • Fonction : Auteur
Judith Burstin

Résumé

Legume seeds are a major source of dietary proteins for humans and animals. Deciphering the genetic control of their accumulation is thus of primary significance towards their improvement. At first, we analysed the genetic variability of the pea seed proteome of three genotypes over 3 years of cultivation. This revealed that seed protein composition variability was under predominant genetic control, with as much as 60% of the spots varying quantitatively among the three genotypes. Then, by combining proteomic and quantitative trait loci (QTL) mapping approaches, we uncovered the genetic architecture of seed proteome variability. Protein quantity loci (PQL) were searched for 525 spots detected on 2-D gels obtained for 157 recombinant inbred lines. Most protein quantity loci mapped in clusters, suggesting that the accumulation of the major storage protein families was under the control of a limited number of loci. While convicilin accumulation was mainly under the control of cis-regulatory regions, vicilins and legumins were controlled by both cis- and trans-regulatory regions. Some loci controlled both seed protein composition and protein content and a locus on LGIIa appears to be a major regulator of protein composition and of protein in vitro digestibility.

Dates et versions

hal-00751802 , version 1 (14-11-2012)

Identifiants

Citer

Michael Bourgeois, Françoise Jacquin, Florence Cassecuelle, Vincent Savois, Maya Belghazi, et al.. A PQL (protein quantity loci) analysis of mature pea seed proteins identifies loci determining seed protein composition.. Proteomics, 2011, 11 (9), pp.1581-94. ⟨10.1002/pmic.201000687⟩. ⟨hal-00751802⟩
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