ClbP is a prototype of a peptidase subgroup involved in biosynthesis of nonribosomal peptides. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2011

ClbP is a prototype of a peptidase subgroup involved in biosynthesis of nonribosomal peptides.

D Dubois
  • Fonction : Auteur
O Baron
  • Fonction : Auteur
A Cougnoux
  • Fonction : Auteur
J Delmas
M Boury
  • Fonction : Auteur
B Bouchon
  • Fonction : Auteur
M Bringer
  • Fonction : Auteur
J Nougayrède
E Oswald
  • Fonction : Auteur
R Bonnet
  • Fonction : Auteur

Résumé

The pks genomic island of Escherichia coli encodes polyketide (PK) and nonribosomal peptide (NRP) synthases that allow assembly of a putative hybrid PK-NRP compound named colibactin that induces DNA double-strand breaks in eukaryotic cells. The pks-encoded machinery harbors an atypical essential protein, ClbP. ClbP crystal structure and mutagenesis experiments revealed a serine-active site and original structural features compatible with peptidase activity, which was detected by biochemical assays. Ten ClbP homologs were identified in silico in NRP genomic islands of closely and distantly related bacterial species. All tested ClbP homologs were able to complement a clbP-deficient E. coli mutant. ClbP is therefore a prototype of a new subfamily of extracytoplasmic peptidases probably involved in the maturation of NRP compounds. Such peptidases will be powerful tools for the manipulation of NRP biosynthetic pathways.
Fichier principal
Vignette du fichier
2011_dubois_j.biol.chem_1.pdf (1.81 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-00748957 , version 1 (29-05-2020)

Licence

Paternité

Identifiants

Citer

D Dubois, O Baron, A Cougnoux, J Delmas, Nathalie Pradel, et al.. ClbP is a prototype of a peptidase subgroup involved in biosynthesis of nonribosomal peptides.. Journal of Biological Chemistry, 2011, 286 (41), pp.35562-70. ⟨10.1074/jbc.M111.221960⟩. ⟨hal-00748957⟩

Collections

IRD UNIV-AMU LMBEC
34 Consultations
52 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More