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Article Dans Une Revue Angewandte Chemie International Edition Année : 2012

Towards the simplification of protein synthesis: iterative solid-supported ligations with concomitant purifications.

Résumé

Please release me: A new linker for the temporary tagging of peptides at their N-terminus after solid-phase elongation, and its potential for capture/release purification is demonstrated. This concept is extended to a remarkably efficient self-purifying N-to-C iterative triazole ligation strategy, which is applied to the synthesis of a polypeptide having 160 residues, in a high purity without the need for chromatographic purification (see picture; orange blocks: peptide segments).

Domaines

Autre [q-bio.OT]

Dates et versions

hal-00748170 , version 1 (05-11-2012)

Identifiants

Citer

Vincent Aucagne, Ibai E Valverde, Philippe Marceau, Mathieu Galibert, Nabil Dendane, et al.. Towards the simplification of protein synthesis: iterative solid-supported ligations with concomitant purifications.. Angewandte Chemie International Edition, 2012, 51 (45), pp.11320-4. ⟨10.1002/anie.201206428⟩. ⟨hal-00748170⟩
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