High-Speed Tracking of Murine Cardiac Stem Cells by Harmonic Nanodoublers.
Résumé
Potassium niobate nonlinear nanoparticles are used for the fi rst time to monitor the evolution of embryonic stem cells (ESC) by second harmonic microscopy. These particles feature the complete absence of photo-bleaching and unlimited excitation wavelength fl exibility. The potential of this approach is made evident for tissueregeneration studies and applications, by capturing a high-speed movie of ESCderived cardiomyocytes autonomously beating within a cluster. Time-resolved data are analyzed to retrieve 3D information of the contraction pattern at the cellular level.