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Article Dans Une Revue Ecotoxicology and Environmental Safety Année : 2011

Differential liver proteome mapping of control and cadmium-fed rats

Résumé

A comparative study of proteome maps from control and Cd-exposed rat liver was performed using a new technology of two-dimensional liquid chromatography separation method (PF-2D system, Beckman Coulter). Rats were fed for one month 0 or 100 μg Cd g(-1). The between-replicate and between-sample variations showed good repeatability and suitable reproducibility for the two dimensions of separation of proteins. In this complex mixture, PF-2D led to the separation of two major peaks which differed between control and Cd-exposed rat livers, one being identified by mass spectrometry as Cu/Zn superoxide dismutase (SOD), a well-known biomarker of Cd exposure, the other as phosphatidylethanolamine binding protein (PEBP). SOD content was decreased in Cd-exposed rat liver, compared to the control group which was corroborated by a significant decrease of SOD activity. PEBP content also tended to be decreased after Cd exposure. Present results demonstrate interest but also limitations of proteomic approach using PF-2D system to analyze effects of chemicals on organisms.

Dates et versions

hal-04596762 , version 1 (31-05-2024)

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Citer

F. Hispard, A. de Vaufleury, C. Schaeffer, R. Scheifler, Pierre-Marie Badot, et al.. Differential liver proteome mapping of control and cadmium-fed rats. Ecotoxicology and Environmental Safety, 2011, 74 (4), pp.576-583. ⟨10.1016/j.ecoenv.2010.07.038⟩. ⟨hal-04596762⟩
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