Differential liver proteome mapping of control and cadmium-fed rats. - Archive ouverte HAL Access content directly
Journal Articles Ecotoxicology and Environmental Safety Year : 2011

Differential liver proteome mapping of control and cadmium-fed rats.

Abstract

A comparative study of proteome maps from control and Cd-exposed rat liver was performed using a new technology of two-dimensional liquid chromatography separation method (PF-2D system, Beckman Coulter). Rats were fed for one month 0 or 100 μg Cd g(-1). The between-replicate and between-sample variations showed good repeatability and suitable reproducibility for the two dimensions of separation of proteins. In this complex mixture, PF-2D led to the separation of two major peaks which differed between control and Cd-exposed rat livers, one being identified by mass spectrometry as Cu/Zn superoxide dismutase (SOD), a well-known biomarker of Cd exposure, the other as phosphatidylethanolamine binding protein (PEBP). SOD content was decreased in Cd-exposed rat liver, compared to the control group which was corroborated by a significant decrease of SOD activity. PEBP content also tended to be decreased after Cd exposure. Present results demonstrate interest but also limitations of proteomic approach using PF-2D system to analyze effects of chemicals on organisms.

Dates and versions

hal-00733934 , version 1 (20-09-2012)

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F. Hispard, Annette De Vaufleury, C. Schaeffer, R. Scheifler, P.-M. Badot, et al.. Differential liver proteome mapping of control and cadmium-fed rats.. Ecotoxicology and Environmental Safety, 2011, 74 (4), pp.576-83. ⟨10.1016/j.ecoenv.2010.07.038⟩. ⟨hal-00733934⟩
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