In recent years, the Quality of Experience (QoE) notion has become a major research theme within the telecommunication community. QoE provides an assessment around a human perception, feeling, performance and behavior. Normally, research studies on multimedia, Quality of Experience (QoE) and gender differences are carried out separately. To develop multimedia solutions covering QoE aspects, keeping the gender differences in mind, are the need of time. In current study, we are interested in knowing how QoE is shaped in Virtual Acoustic Environment (VAE) for male and female. We present experimental test results which provide interesting findings that both male and female have difference in their performance and perception in locating concurrent talkers in small and big sized virtual conferencing rooms. The middle-sized virtual room was suitable for teleconferencing because both male and female participants' performance and perception converge to similar trend and obtained better QoE values.