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Article Dans Une Revue Small Année : 2012

One Step Synthesis of Gold-Loaded Radial Mesoporous Silica Nanospheres and Supported Lipid Bilayer Functionalization: Towards Bio-Multifunctional Sensors

Résumé

A simple synthetic route is developed to achieve gold functionalized radial mesoporous silica nanoparticles (Au-MsNP) synthesized by a one step procedure fully compatible with basic conditions required for the preparation of monodispersed nanospheres. In a second step, Au-MsNP particles have been coated with phospholipid bilayers in order to design an advanced biofunctional platform with the gold metallic nanoparticles previously grown into the pore channels and responsible for a plasmonic activity relevant for biosensing. The size of Au-MsNP is checked by dynamic light scattering while zeta potential measurements reflect their surface charge. The particle morphology is characterized by transmission and scanning electron microscopy and the Si/Au ratios are obtained from energy dispersive X-ray analysis. The textural properties of Au-MsNP, specific surface area and pore size, are determined from N2 adsorption. The supported bilayers are achieved from vesicles of different phospholipids incubated with Au-MsNP particles. The coating efficiency is investigated by zeta potential and cryo- transmission electron microscopy. The plasmonic activities of bare Au-MsNP particles and coated lipid bilayer Au-MsNP platform are evidenced for two model systems: direct adsorption of bovine serum albumin and molecular recognition events between avidin molecules and biotin receptors integrated in the supported lipid bilayer.

Dates et versions

hal-00731463 , version 1 (12-09-2012)

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Citer

Remi Veneziano, Gaëlle Derrien, Sisareuth Tan, A. Brisson, Jean-Marie Devoisselle, et al.. One Step Synthesis of Gold-Loaded Radial Mesoporous Silica Nanospheres and Supported Lipid Bilayer Functionalization: Towards Bio-Multifunctional Sensors. Small, 2012, 8 (23), pp.3674-3682. ⟨10.1002/smll.201200758⟩. ⟨hal-00731463⟩
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