Article Dans Une Revue Journal of Spectroscopy Année : 2012

A new method for D2O/H2O exchange in infrared spectroscopy of proteins

Résumé

In this paper, we describe a new method to obtain D2O/H2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides. The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.

Dates et versions

hal-00725336 , version 1 (24-08-2012)

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Citer

M. Malferrari, G. Venturoli, F. Francia, A. Mezzetti. A new method for D2O/H2O exchange in infrared spectroscopy of proteins. Journal of Spectroscopy, 2012, 27, pp.337 - 342. ⟨10.1155/2012/791342⟩. ⟨hal-00725336⟩
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