The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue EMBO Journal Année : 2011

The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators.

Résumé

In Escherichia coli, the essential motor protein Rho promotes transcription termination in a tightly controlled manner that is not fully understood. Here, we show that the general post-transcriptional regulatory protein Hfq associates with Rho to regulate Rho function. The Hfq:Rho complex can be further stabilized by RNA bridging both factors in a configuration that inhibits the ATP hydrolysis and duplex unwinding activities of Rho and that mediates transcription antitermination at Rho-dependent terminators in vitro and in vivo. Antitermination at a prototypical terminator (λtR1) requires Hfq binding to an A/U-rich transcript region directly upstream from the terminator. Antitermination is modulated by trans-acting factors (NusG or nucleic acid competitors) that affect Hfq association with Rho or RNA. These data unveil a new Hfq function and a novel transcription regulatory mechanism with potentially important implications for bacterial RNA metabolism, gene silencing, and pathogenicity.

Dates et versions

hal-00720713 , version 1 (25-07-2012)

Identifiants

Citer

Makhlouf Rabhi, Olivier Espéli, Annie Schwartz, Bastien Cayrol, a Rachid Rahmouni, et al.. The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators.. EMBO Journal, 2011, 30 (14), pp.2805-16. ⟨10.1038/emboj.2011.192⟩. ⟨hal-00720713⟩
84 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More