Atmospheric pressure photoionization as a powerful tool for large-scale lipidomic studies. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of The American Society for Mass Spectrometry Année : 2012

Atmospheric pressure photoionization as a powerful tool for large-scale lipidomic studies.

Mathieu Gaudin
  • Fonction : Auteur
Laurent Imbert
Danielle Libong
  • Fonction : Auteur
Pierre Chaminade
David Touboul

Résumé

Lipidomic studies often use liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) for separation, identification, and quantification. However, due to the wide structural diversity of lipids, the most apolar part of the lipidome is often detected with low sensitivity in ESI. Atmospheric pressure (APPI) can be an alternative ionization source since normal-phase solvents are known to enhance photoionization of these classes. In this paper, we intend to show the efficiency of APPI to identify different lipid classes, with a special interest on sphingolipids. In-source APPI fragmentation appears to be an added value for the structural analysis of lipids. It provides a detailed characterization of both the polar head and the non polar moiety of most lipid classes, and it makes possible the detection of all lipids in both polarities, which is not always possible with ESI.

Domaines

Chimie organique

Dates et versions

hal-00690894 , version 1 (24-04-2012)

Identifiants

Citer

Mathieu Gaudin, Laurent Imbert, Danielle Libong, Pierre Chaminade, Alain Brunelle, et al.. Atmospheric pressure photoionization as a powerful tool for large-scale lipidomic studies.. Journal of The American Society for Mass Spectrometry, 2012, 23 (5), pp.869-79. ⟨10.1007/s13361-012-0341-y⟩. ⟨hal-00690894⟩
34 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More