Our recent studies showed that solid phase phospholipid bilayers play an important role in joint lubrication mechanism. We investigate the tribological properties of fluid phase bilayers depending on various experimental parameters. Bilayers containing a fluorescent lipid with a large head-group and surfaces covered with only one bilayer poorly lubricate meaning that the slip plane during shear is located in the thin aqueous layer between the bilayers. Lubrication and resistance to normal and tangential stresses are greatly improved in buffered saline solution. Bilayers prepared by vesicle fusion method improve the tribological properties of even very rough substrates by smoothing their topographical defects.