EGR1 expression: a calcium and ERK1/2 mediated PPAR-independent event involved in the antiproliferative effect of 15-deoxy-(12,14-prostaglandin J2 and Thiazolidinediones in breast cancer cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Pharmacology Année : 2011

EGR1 expression: a calcium and ERK1/2 mediated PPAR-independent event involved in the antiproliferative effect of 15-deoxy-(12,14-prostaglandin J2 and Thiazolidinediones in breast cancer cells

Sarra Chbicheb
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Xiao Yao
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Stéphane Salamone
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Michel Boisbrun
Daniel Spohn
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Isabelle Grillier-Vuissoz
Yves Chapleur
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Stéphane Flament
Sabine Mazerbourg
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Résumé

Our aim was to get new information about the Peroxisome Proliferator Activated Receptor gamma (PPARγ)-independent pathway involved in the antiproliferative action of PPARγ ligands in breast cancer cells. We investigated the effects of Troglitazone (TGZ), Ciglitazone (CGZ), Rosiglitazone (RGZ) and, 15-deoxy-(12,14-prostaglandin J2 (15d-PGJ) on the hormone-dependent breast cancer cell line MCF7. The early transcription factor (Early Growth Response gene 1) mRNA and protein levels peaked after 3hours of incubation with 25μM TGZ, CGZ or 15d-PGJ and then gradually decreased. RGZ, the most potent activator of PPARγ, did not show this effect. The PPARγ antagonist GW 9662 did not block mRNA induction which also still occurred in case of PPARγ silencing as well as in case of treatment with the PPARγ-inactive compound Δ2-TGZ. mRNA induction required ERK1/2 phosphorylation which was not blocked by EGF Receptor (EGFR) inhibition. The ERK1/2 pathway was also involved in Δ2-TGZ-induced mRNA expression in the hormone-independent breast cancer cell line MDA-MB-231. Using the fluorescent dye Fura2, we showed in MCF7 that TGZ or Δ2-TGZ induced an immediate increase in cytosolic calcium which was required for ERK1/2 phosphorylation and mRNA induction as demonstrated by calcium chelation experiments. Furthermore, in MCF7 transfected with siRNA targeting Δ2-TGZ inhibited less efficiently cell proliferation.

Domaines

Pharmacologie
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Dates et versions

hal-00685074 , version 1 (04-04-2012)

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Sarra Chbicheb, Xiao Yao, Jean-Luc Rodeau, Stéphane Salamone, Michel Boisbrun, et al.. EGR1 expression: a calcium and ERK1/2 mediated PPAR-independent event involved in the antiproliferative effect of 15-deoxy-(12,14-prostaglandin J2 and Thiazolidinediones in breast cancer cells. Biochemical Pharmacology, 2011, 81 (9), pp.1087. ⟨10.1016/j.bcp.2011.02.006⟩. ⟨hal-00685074⟩
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