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Article Dans Une Revue ChemBioChem Année : 2008

Peptide-grafted nanodiamonds: preparation, cytotoxicity and uptake in cells.

Résumé

Nanodiamonds that were prepared by high pressure/high temperature were functionalized with biomolecules for biological applications. Nanodiamonds (NDs, < or =35 nm) that were coated by silanization or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.

Domaines

Chimie organique

Dates et versions

hal-00662943 , version 1 (25-01-2012)

Identifiants

Citer

Stéphanie Vial, Christelle Mansuy, Sandrine Sagan, Theano Irinopoulou, Fabienne Burlina, et al.. Peptide-grafted nanodiamonds: preparation, cytotoxicity and uptake in cells.. ChemBioChem, 2008, 9 ((13)), pp.2113-9. ⟨10.1002/cbic.200800247⟩. ⟨hal-00662943⟩
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