Immobilization of double functionnalized carbon nanotubes on glassy carbon electrodes for the electrochemical sensing of the biotin-avidin affinity - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of electroanalytical chemistry and interfacial electrochemistry Année : 2012

Immobilization of double functionnalized carbon nanotubes on glassy carbon electrodes for the electrochemical sensing of the biotin-avidin affinity

Résumé

Multi-walled carbon nanotubes (MWCNTs) double functionalized with redox-active ferrocene and biotin (Fc-Biot-MWCNTs) were synthesized and used for the electrochemical detection of avidin. After dispersion in perfluorosulfonated polymer Nafion and immobilization on the electrode surfaces, the cyclic voltammetry response of the modified electrodes showed in aqueous medium a quasi-reversible one-electron system at 0.46 V vs. SCE, assigned to the bound ferrocene/ferrocenium redox couple. Upon the addition of avidin in the range 0.9-20 nM, a stepwise decrease of both anodic and cathodic peak currents ascribed to the ferrocene was observed. These electrochemical changes are specifically due to the formation of biotin-avidin complex and are explained by complexation-induced modifications in the environment of covalently bound ferrocene.
Fichier non déposé

Dates et versions

hal-00658205 , version 1 (10-01-2012)

Identifiants

Citer

Bruno Fabre, Cristian Samori, Alberto Bianco. Immobilization of double functionnalized carbon nanotubes on glassy carbon electrodes for the electrochemical sensing of the biotin-avidin affinity. Journal of electroanalytical chemistry and interfacial electrochemistry, 2012, 665, pp.90-94. ⟨10.1016/j.elechem.2011.11.029⟩. ⟨hal-00658205⟩
113 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More