Comparative Evaluation of the Seegene Seeplex RV15 and Real-Time PCR for Respiratory Virus Detection
Résumé
Respiratory virus infections contribute substantially both to hospitalisations of young children, and to morbidity in immunocompromised patients such as those with haemotological malignancies. Their rapid and accurate diagnosis is essential to patient management. To evaluate the prospective utility of Seeplex® DPO technology in respiratory virus diagnosis, a panel of 99 respiratory samples positive by real-time PCR for one or more viruses was assayed by the Seegene Seeplex® RV12 system. As well as being able to detect all ten viruses in the RT-PCR system with the exception of enteroviruses, RV12 can also distinguish between the two subgroups of RSV and detect two subgroups of coronaviruses. Seven of the 9 viruses in common with the RT-PCR were reliably detected by RV12. Furthermore, with each virus, some negative correlation was observed between RT-PCR threshold cycle and Seeplex® peak intensity, suggesting the latter could also be employed semi-quantitatively. Eleven samples RT-PCR-positive for Metapneumovirus and 5 samples positive for Influenza B were not detected by RV12. Seegene developed a second-generation system, RV15, which not only allowed detection of 3 additional viruses, but also addressed the potential problems with RV12 specificity. To address these concerns, 84 respiratory samples positive for a range of viruses by real-time PCR were assayed with RV15. The results of this evaluation improved significantly upon those seen with RV12. The high throughput capabilities and potential lower technical requirements afforded by the Seeplex® system may offer an alternative to real-time RT-PCR systems
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