False-negative test results in the Slidex Staph Plus (bioMérieux) agglutination test are mainly caused by -type t001 and t001-related strains - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue European Journal of Clinical Microbiology and Infectious Diseases Année : 2010

False-negative test results in the Slidex Staph Plus (bioMérieux) agglutination test are mainly caused by -type t001 and t001-related strains

Résumé

The relative sensitivity of commercial agglutination kits for fast identification of is usually given to be about 98%. This reported sensitivity has sometimes been questioned. In this study, three collections of molecularly defined, single-copy strains of were used to compare the sensitivities of agglutination-based identification and the MALDI-TOF mass spectrometry-based identification using the Biotyper 2.0 database to a molecularly defined reference method. Clinical isolates ( = 363) of methicillin-susceptible (MSSA) and 240 clinical isolates of methicillin-resistant (MRSA) were included. In order to rule out a predominance of local MRSA-strains, a collection of 104 pulsed-field-gel electrophoresis divergent MRSA strains were also tested. MALDI-TOF MS using Biotyper database (Bruker) identified all isolates, whereas the Slidex Staph Plus (bioMérieux) detected only 98.0% of the MSSA, 94.5% of the MRSA and only 70.1% of the MRSA of the molecularly divergent strain collection. Interestingly, strains with a false-negative test result in the agglutination methods were mostly -type t001 and t001 related. The MALDI-TOF MS based identification can thus be used as an alternative identification method for suspected false-negative results from the agglutination tests, especially if the local prevalence of t001 and t001 related strains is high.

Mots clés

Fichier principal
Vignette du fichier
PEER_stage2_10.1007%2Fs10096-010-1070-4.pdf (174.25 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-00630325 , version 1 (09-10-2011)

Identifiants

Citer

Florian Szabados, J. Woloszyn, M. Kaase, S. G. Gatermann. False-negative test results in the Slidex Staph Plus (bioMérieux) agglutination test are mainly caused by -type t001 and t001-related strains. European Journal of Clinical Microbiology and Infectious Diseases, 2010, 30 (2), pp.201-208. ⟨10.1007/s10096-010-1070-4⟩. ⟨hal-00630325⟩

Collections

PEER
34 Consultations
480 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More